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Updated: Aug 13, 2026
Preparing T Cell Growth Factor from Rat Splenocytes
Published on: October 31, 2007
[RDP1258 on proliferation of rat splenocytes in vivo]
Shan-hong Yi1, Gang Ye, Quan Feng
1Department of Urology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. yshh881@sohu.com
Aim:
To explore the immunosuppressive effects of a novel HLA-derived peptide, RDP1258, on rat splenocyte proliferation in vivo and its underlying mechanisms.
Methods:
RDP1258 was synthesized through artificial solid-phase synthesis method. Its effects on proliferative reaction of rat splenocytes in vivo were observed by (3)H-TDR incorporation method. And its effects on the heme oxygenase(HO) enzyme activity were analyzed by enzyme chemistry method.
Results:
The results showed that the synthetic HLA-derived peptide RDP1258 obviously inhibited the proliferation of rat splenocytes stimulated by isoantigen and mitogen. In vivo administration of RDP1258 peptide to rats resulted in upregulation of splenic HO activity and rapid upregulation of HO-1 protein expression.
Conclusion:
HO-1 might participate in RDP1258 inhibiting the proliferation of rat splenocytes induced by mitogen and isoantigen in vivo.
