Related Experiment Video
Updated: Aug 12, 2026

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
Published on: September 10, 2017
Indirect chemiluminescence detection for capillary zone electrophoresis of monoamines and catechol using
Yonggang Hu1, Xinxin Li, Zhitao Pang
1College of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. yongganghu@163.com
Abstract:
Indirect chemiluminescence (ICL) detection for capillary electrophoresis (CE) of monoamines and catechol using luminol-K3 [Fe(CN)6] system was described. A strong and stable background chemiluminescence (CL) signal can be generated by luminol-K3 [Fe(CN)6] reaction. Based on the principle of that some phenolic compounds may be oxidized in the presence of K3 [Fe(CN)6], quenching effect of catecholamines for luminol-K3[Fe(CN)6] CL reaction results in a quantifiable decrease in the background signal. The conditions for CE separation and the CL detection for four standard catecholamines were systematically investigated using a homemade CE-ICL system. Under the optimum conditions, the detection limits of dopamine (DA), epinephrine (EP), norepinephrine (NE) and catechol (CA) were determined to be 0.18 mciroM 0.39 microM 0.48 microM and 0.09 microM, respectively. It also has been successfully applied to analyze seven pharmaceutical samples and seven human urine samples.
Related Concept Videos
Gas Chromatography: Types of Detectors-II
High-Performance Liquid Chromatography: Types of Detectors
Capillary Electrophoresis: Instrumentation
Photoluminescence: Applications

