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Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes
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Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

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Identification of Hub Genes Correlated with the Initiation and Development in Chronic Kidney Disease via

Kai Zhu1,2, Xinxin Li3, Likun Gao4

  • 1Department of Nephrology, Renmin Hospital of Wuhan University, Wuhan, China, 13484200@qq.com.

Kidney & Blood Pressure Research
|January 5, 2023
PubMed
Abstract

Insights

This study investigated the molecular mechanisms of chronic kidney disease (CKD) using gene expression analysis in mice. Key genes like GNG2 and PF4 were identified as potential therapeutic targets for CKD treatment.

Area of Science:

  • Nephrology
  • Molecular Biology
  • Genomics

Background:

  • Chronic kidney disease (CKD) presents a significant global health challenge, marked by irreversible nephron loss and declining renal function.
  • The intricate molecular pathways driving CKD progression remain incompletely understood.

Purpose of the Study:

  • To elucidate the molecular mechanisms underlying CKD.
  • To identify key genes and pathways involved in the pathogenesis of CKD.

Main Methods:

  • Integrated three transcriptional profile datasets to identify differentially expressed genes (DEGs) in a mouse model of unilateral ureteral obstruction (UUO).
  • Utilized bioinformatics tools including limma, Gene Ontology, KEGG pathway analysis, STRING database, MCODE, and Cytohubba for gene and protein-protein interaction network analysis.
  • Validated gene expression changes in an independent dataset and a mouse UUO model.

Main Results:

  • Identified 315 shared DEGs between control and UUO samples, enriched in inflammatory response, immune processes, and chemokine signaling.
  • Protein-protein interaction network analysis revealed modules related to macrophage activation and identified ten hub genes.
  • Validated four hub genes, with GNG2, PF4, and CCL9 showing significant response to UUO.

Conclusions:

  • The study highlights coordinated gene expression during UUO, offering a potential gene panel for CKD therapeutic strategies.
  • GNG2 and PF4 emerge as promising molecular targets for the development of novel CKD therapeutics.
Keywords:
Bioinformatic analysisChronic kidney diseaseDifferentially expressed genesHub genesProtein-protein interactionUnilateral ureteral obstruction

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