Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Radioreceptor assay for human chorionic gonadotropin.

C Y Lee, R J Ryan

    The Journal of Clinical Endocrinology and Metabolism
    |February 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effect of hyperthermia on intracellular pH in human U-87 MG glioblastoma cells.

    Oncology·1995
    Same author

    Purified rat acid-labile subunit and recombinant human insulin-like growth factor (IGF)-binding protein-3 can form a 150-kilodalton binary complex in vitro in the absence of IGFs.

    Endocrinology·1995
    Same author

    Molecular analysis of an extracellular protease gene from Vibrio parahaemolyticus.

    Microbiology (Reading, England)·1995
    Same author

    Set-shifting aptitude in Parkinson's disease: external versus internal cues.

    Psychological reports·1995
    Same author

    Luminal epidermal growth factor is trophic to the small intestine of parenterally fed rats.

    Clinical science (London, England : 1979)·1995
    Same author

    Comparative mathematical analyses of freezing in lung and solid tissue.

    Cryobiology·1995

    A new radioreceptor assay accurately detects human chorionic gonadotropin (hCG) in urine and serum. This sensitive method offers a rapid and simple way to measure biologically active hCG, correlating well with pregnancy tests.

    Area of Science:

    • Endocrinology
    • Biochemistry
    • Immunoassay Development

    Background:

    • Human chorionic gonadotropin (hCG) is a crucial hormone for pregnancy detection and monitoring.
    • Existing radioimmunoassays (RIAs) for hCG have limitations in measuring biological activity.
    • Development of sensitive and specific assays for hCG is vital for reproductive health diagnostics.

    Purpose of the Study:

    • To develop a sensitive radioreceptor assay (RRA) for quantifying human chorionic gonadotropin (hCG).
    • To compare the performance of the RRA with established radioimmunoassays (RIAs) and pregnancy tests.
    • To assess the utility of the RRA for measuring biologically active hCG in clinical samples.

    Main Methods:

    • Development of a radioreceptor assay using ovarian receptors and radiolabeled hCG ((125I)hCG).

    Related Experiment Videos

  • Analysis of dose-response curves for urine and serum samples from pregnant women against standard hCG curves.
  • Comparison of potency estimates from RRA with those from RIA and correlation with agglutination pregnancy tests.

    • Main Results:

    • The developed RRA is sensitive, detecting as little as 100 pg of hCG.
    • Dose-response curves in clinical samples were linear and parallel to standard curves.
    • RRA potency estimates for pregnancy sera were approximately twofold higher than RIA, indicating measurement of greater biological activity.
    • Urine hCG concentrations measured by RRA correlated well with results from standard agglutination pregnancy tests.

    Conclusions:

    • The RRA provides a sensitive, rapid, and simple method for measuring biologically active hCG.
    • This assay can achieve sensitivity comparable to radioimmunoassays.
    • The RRA is a valuable tool for assessing hCG and luteinizing hormone (LH) levels in clinical diagnostics.