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Published on: May 4, 2021
Effect of nitric oxide and peroxynitrite on mucociliary transport function of experimental otitis media
Eun-ju Jeon1, Yong-Soo Park, Seung Kyun Lee
1Department of Otolaryngology-HNS, College of Medicine, The Catholic University of Korea, Our Lady of Mercy Hospital, 665 Bupyung-dong, Bupyung-gu, Incheon 403-720, Korea.
Objective:
To determine the role of nitric oxide (NO) and peroxynitrite on mucociliary activity in experimental otitis media with effusion (OME).
Study Design And Setting:
Twenty guinea pigs were divided into 1 control and 3 experimental groups; lipopolysaccharide (LPS), N(G)-nitro-L-arginine methyl ester (L-NAME), and uric acid (UA) groups. Ten ears were used in each group. OME was induced by transtympanic injection of LPS in experimental groups. Twenty-four hours after the transtympanic injection, dye transfer time (DTT) was measured and the temporal bone was taken for histopathologic examination. Expression of peroxynitrite was determined by immunohistochemical stain for 3-nitrotyrosine (3-NT).
Results:
Dye transfer time was significantly delayed in LPS group compared to control group; by contrast it was significantly reduced in L-NAME or UA treated groups (P < 0.01). Histopathologic examination showed reduced inflammation and mucosal thickening in the treatment groups when compared to LPS group. These findings, however, were not statistically significant. Immunoreactivity to 3-NT was intense in LPS group and decreased in the treatment groups (P < 0.05).
Conclusions:
It is suggested that LPS induced mucociliary dysfunction in the middle ear by NO and peroxynitrite-mediated pathways.
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