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Stripping interfering sugars from samples using adapted bacteria.

I N Boe¹, R E Lovrien, D W Karl

¹Biochemistry Department, Gortner Laboratory, University of Minnesota, St. Paul 55108.

Journal of Biochemical and Biophysical Methods

|June 1, 1992

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Adapted bacteria efficiently remove specific sugars from samples, acting as precise reagents for sugar analysis. This simple bacterial stripping method removes interfering sugars down to micromolar concentrations.

Area of Science:

Microbiology
Biochemistry
Analytical Chemistry

Background:

Interference from background sugars can complicate the analysis of target sugars.
Existing methods for sugar removal may be complex or inefficient.
Bacteria possess metabolic pathways for sugar uptake and utilization.

Purpose of the Study:

To develop a simple and specific method for removing interfering sugars from analytical samples.
To explore the use of adapted bacteria as selective reagents for sugar stripping.
To determine the efficiency and conditions for bacterial sugar removal.

Main Methods:

Bacteria, such as Escherichia coli and Klebsiella, were adapted to specific sugars by growth.
Adapted bacterial cells were used to 'strip' target sugars from complex mixtures.

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The process involved mixing cells with the sample, centrifugation, and collection of the supernatant.

Main Results:

Adapted bacteria effectively removed specific sugars down to micromolar and submicromolar concentrations (bacterial Km values).
Bacterial stripping is efficient for samples with high background sugar concentrations (millimolar to larger).
The rapid 1-5 minute stripping process prevents sugar metabolism and metabolite leakage.

Conclusions:

Bacterial stripping is a simple, rapid, and specific method for removing interfering sugars.
Adapted bacteria serve as effective, reusable reagents for pre-analytical sample cleanup.
This technique enhances the accuracy of sought-for sugar analysis in complex matrices.