Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Temperature-programmed assembly of DNA:Au nanoparticle bioconjugates.

Lisa M Dillenback1, Glenn P Goodrich, Christine D Keating

  • 1Department of Chemistry, The Pennsylvania State University, University Park, 16802, USA.

Nano Letters
|January 13, 2006
PubMed
Summary

Temperature programming controls DNA strand hybridization for ordered nanoparticle assembly. This method enables precise construction of complex nanostructures by selectively forming DNA duplexes at specific temperatures.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic and Catalytic Multiphase Coacervates.

Biomacromolecules·2026
Same author

Increasing the Compositional Heterogeneity of Single-Chain Amphiphile Membranes Supported by Coacervate Cores Alters Stability and Properties of the Hybrid Protocells.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Residue-level mapping of crowding effects on protein phase separation.

Protein science : a publication of the Protein Society·2026
Same author

From Polymerization-Enabled Folding and Assembly to Chemical Evolution: Key Processes for Emergence of Functional Polymers in the Origin of Life.

Astrobiology·2025
Same author

Primitive Molecular Buffering by Low-Multivalency Coacervates.

The journal of physical chemistry. B·2025
Same author

Hybrid Protocells Based on Coacervate-Templated Fatty Acid Vesicles Combine Improved Membrane Stability with Functional Interior Protocytoplasm.

Small (Weinheim an der Bergstrasse, Germany)·2024

Area of Science:

  • Nanotechnology
  • Biotechnology
  • Materials Science

Background:

  • DNA-directed assembly offers precise control over nanoparticle arrangement.
  • Controlling the order of hybridization for multiple DNA strands in solution is challenging.
  • Gold nanoparticles functionalized with thiolated DNA are used for self-assembly.

Purpose of the Study:

  • To investigate the use of temperature programming to control the order of DNA hybridization events.
  • To demonstrate the synthesis of complex nanostructured materials using controlled assembly.
  • To assess the impact of DNA strand loss and exchange on assembly order.

Main Methods:

  • Utilizing temperature gradients to selectively form DNA duplexes based on thermal stability.
  • Employing thiolated DNA on gold nanoparticles for self-assembly in solution.

Related Experiment Videos

  • Monitoring assembly processes at varying temperatures to control hybridization sequence.

    • Main Results:

    • Higher temperatures favored the formation of more thermally stable DNA duplexes.
    • A stepwise cooling process allowed for sequential hybridization of different DNA sequences.
    • Despite some DNA strand loss at elevated temperatures, cooperativity ensured the formation of correct assemblies.
    • The order of assembly was successfully controlled using temperature programming.

    Conclusions:

    • Temperature programming is an effective strategy to dictate the order of DNA hybridization in nanoparticle assembly.
    • This method enhances the synthesis of complex nanostructured materials by controlling assembly sequences.
    • The approach combines DNA-directed assembly selectivity with precise temporal control over molecular interactions.