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Response of different organs of immune system of mice upon administration bacterial CpG DNA
S Olishevsky1, V Shlyakhovenko, V Kozak
1R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine. sergeyolishevsky@yahoo.com
Unlabelled:
CpG DNA are potent immunostimulator and currently being tested as adjuvant in immunotherapy of various diseases. THE AIM of this study was to investigate the effects of a single dose of bacterial CpG DNA challenge on response of murine immune system organs and on a zeta-potential of different lymphoid organ cells and peritoneal macrophages in normal and tumor-bearing mice.
Methods:
Indexes and cellularity of immune organs of mice were evaluated. Z-potential was measured by cellular electrophoresis.
Results:
Subcutaneous administration of CpG DNA induced local transitory hyperplasia of lymph nodes, moderate increase of splenic index and total spleen cellularity, whereas intraperitoneal injection of CpG DNA caused full-blown spleen enlargement and increase of splenocyte content, and also mice showed transitory aseptic peritonitis. Response of thymus on challenge of CpG DNA was bi-phase: initial phase - response on antigen as stimulus, and late proliferative phase. It was noted that tumor growth does not affect zeta-potential in peritoneal macrophages and mononuclear lymphocytes, but causes increase of zeta-potential on thymocytes and decreases it in lymphocytes from lymph nodes. Furthermore, single administration of CpG DNA normalizes of thymocytes and lymph nodes lymphocytes zeta-potential and increases it in peritoneal macrophages and mononuclear lymphocytes.
Conclusion:
The findings demonstrate a close correlation between the hyperplasia of lymphoid follicles induced by challenge of CpG DNA and increase of their cellularity. Observed Z-potential alterations of immune system cells after CpG DNA immunization evidence on more significant polyanion accumulation on the surface of splenic macrophages and mononuclear cells.
Insights
Bacterial CpG DNA, a potent immune stimulant, alters immune organ responses and cell surface charge (zeta-potential) in mice. This study reveals CpG DNA normalizes zeta-potential in certain immune cells, enhancing immunotherapy potential.
Area of Science:
- Immunology
- Molecular Biology
- Cancer Research
Background:
- CpG DNA acts as a powerful immunostimulant, explored as an adjuvant in various immunotherapies.
- Understanding CpG DNA's impact on immune organs and cell surface properties is crucial for optimizing its therapeutic use.
Purpose of the Study:
- To investigate the effects of a single bacterial CpG DNA dose on murine immune system organs.
- To analyze alterations in zeta-potential of lymphoid organ cells and peritoneal macrophages in normal and tumor-bearing mice.
Main Methods:
- Evaluation of immune organ indexes and cellularity in mice.
- Measurement of zeta-potential using cellular electrophoresis.
Main Results:
- CpG DNA administration induced immune organ hyperplasia and increased cellularity, varying with administration route (subcutaneous vs. intraperitoneal).
- Tumor growth affected zeta-potential in thymocytes and lymph node lymphocytes, but not peritoneal macrophages or mononuclear lymphocytes.
- CpG DNA administration normalized zeta-potential in thymocytes and lymph node lymphocytes while increasing it in peritoneal macrophages and mononuclear lymphocytes.
Conclusions:
- CpG DNA challenge correlates with lymphoid follicle hyperplasia and increased cellularity.
- Observed zeta-potential alterations indicate increased polyanion accumulation on splenic macrophages and mononuclear cells following CpG DNA immunization.
