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Updated: Aug 13, 2026
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
Published on: June 8, 2020
[Study on differential proteomic expression in human liver cells stimulated by trichloroethylene with proteomics]
Jian-jun Liu1, Hai-yan Huang, Zhi-xiong Zhuang
1Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.
Objective:
To explore the differential proteomic expression in human liver cells L-02 induced by different dosages of trichloroethylene (TCE).
Methods:
Human liver cells L-02 were treated with different concentrations of TCE and the solvent control (dimethylsulfoxide). The total cellular proteins were separated using 2DE and visualized with silver staining after TCE treatment. The images were analyzed with Image Master 2D Platinum 5.0 analysis software. The differentially expressed protein spots were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-TOF-MS).
Results:
Fifteen protein spots with significant difference were found, and went upward or downward or disappeared after the stimulation of TCE with different dosages, which indicated that TCE induced the change of the proteomic expression in the liver cells. The mass spectrum identification and the IPI human database retrieval were used for identifying 9 proteins related to the L-02 Liver cells induced by TCE.
Conclusion:
The result provides an insight to TCE-related molecular mechanism and which might be useful for further study of the TCE-associated proteins and molecular markers.
