Related Experiment Video
Updated: Aug 13, 2026
Determining Four Components in a Lipid Nanoparticle RNA Delivery System by Liquid Chromatography Combined with Evaporative Light Scattering Detector
Published on: May 30, 2025
The determination of nicotinic acid in plasma by mixed-mode liquid chromatography-tandem mass spectrometry following
D N Mallett1, S Dayal, G J Dear
1Drug Metabolism and Pharmacokinetics, Cardiovascular and Urogenital Centre of Excellence in Drug Discovery, GlaxoSmithKline R&D, Gunnels Wood Road, Stevenage, Herts, UK. dave.n.mallett@gsk.com
Abstract:
An assay for nicotinic acid in plasma samples has been developed using ion exchange solid phase extraction in 96-well format followed by mixed-mode ion exchange/reversed-phase liquid chromatography with positive ion tandem mass spectrometry detection. The assay avoids the need for time-consuming derivatisation procedures or involatile ion-pair chromatography reagents. The assay is linear over the wide range 0.05-20 microg/mL, based on a 100 microL sample (correlation coefficient>0.99). The assay is accurate and precise (bias and coefficient of variation<18%) over this calibration range.
More Related Videos
08:56Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
Published on: November 22, 2024
09:36Applications of Liquid-Chromatography Tandem Mass Spectrometry in Natural Products Research: Tropane Alkaloids as a Case Study
Published on: March 8, 2024
