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Published on: October 21, 2017
[Changes of gene expression profiles in CCl4 injured liver of mice]
Yong Chen1, Ming Cheng, Qi-Song Xia
1Hubei Provincial Key Laboratory of Biotechnology of Traditional Chinese Medicine, Hubei University, Wuhan 430062, China. cy101610@npc.gov.cn
Aim:
To study the gene expression profiles between the CCl4 injured liver and normal liver in mice, and to screen the differentially expressed genes that relate to liver injury by CCl4 on a large scale using cDNA microarrays.
Methods:
Male Kunming strain mice were divided into two groups: one was control group and another was CCl4 injured liver group that was given 0.1% CCI4 oil solution ip at dose of 10 mL x kg(-1) every three days, totally for ten times. Then mRNA in livers of the two groups of mice was extracted, separately, and reversely transcribed to cDNA with the incorporation of different fluorescent-labeled dUTP as the hybridization probes. The mixed probes were hybridized to the cDNA microarrays. The fluorescent signal values were acquired by scanner and analyzed with statistical software.
Results:
Among the 14 100 target genes, 379 genes were differentially expressed, in which 163 genes were up-regulated and the other 216 genes were down-regulated. They are closely related to a range of biological functions.
Conclusion:
Using the cDNA microarray and experimental animal modeling technique, the differentially expressed genes of CCl4 injured liver in mice on a large scale could be studied. It is useful for further investigation of the injury mechanism of CCl4.
Insights
This study used cDNA microarrays to identify gene expression changes in carbon tetrachloride (CCl4) injured mouse livers. Researchers found 379 differentially expressed genes, offering insights into liver injury mechanisms.
Area of Science:
- Molecular Biology
- Toxicology
- Genomics
Background:
- Carbon tetrachloride (CCl4) is a common hepatotoxin used to induce liver injury in experimental models.
- Understanding the molecular mechanisms of CCl4-induced liver injury is crucial for developing therapeutic strategies.
Purpose of the Study:
- To comprehensively analyze gene expression profiles in CCl4-induced liver injury in mice.
- To identify differentially expressed genes associated with CCl4-induced hepatotoxicity using large-scale screening.
Main Methods:
- Male Kunming mice were subjected to CCl4 administration or served as controls.
- Messenger RNA (mRNA) was extracted from liver tissues and converted to complementary DNA (cDNA) probes.
- cDNA microarrays were hybridized with fluorescent-labeled probes, and data were analyzed using statistical software.
Main Results:
- Out of 14,100 genes analyzed, 379 showed differential expression in CCl4-injured livers compared to controls.
- Specifically, 163 genes were upregulated, and 216 genes were downregulated.
- These differentially expressed genes are involved in various biological functions relevant to liver injury.
Conclusions:
- cDNA microarray technology enables large-scale identification of differentially expressed genes in CCl4-induced liver injury.
- The identified genes provide a valuable resource for further research into the mechanisms of CCl4 hepatotoxicity.
