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Updated: Aug 13, 2026
14:47
Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
Published on: May 17, 2016
p14ARF directly interacts with Myc through the Myc BoxII domain
Stefano Amente1, Barbara Gargano, Francesca Varrone
1Department of Structural and Functional Biology, University of Naples "Federico II", Naples, Italy.
Cancer Biology & Therapy
|January 18, 2006
Summary
Human p14ARF protein binds to the Myc proto-oncogene, relocating it within the cell. This interaction downregulates Myc-activated transcription, impacting cell growth regulation.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Myc is a proto-oncogene regulating cell proliferation.
- ARF tumor suppressor gene activates p53 and has p53-independent functions.
- Mouse p19ARF negatively regulates Myc activity.
Purpose of the Study:
- Investigate the interaction between human p14ARF and Myc.
- Determine the mechanism by which p14ARF affects Myc activity.
- Elucidate the role of p14ARF in regulating Myc-driven transcription.
Main Methods:
- Protein interaction assays (in vitro and in vivo).
- Immunofluorescence microscopy for protein colocalization.
- Analysis of Myc-activated transcription levels.
Main Results:
- Human p14ARF directly associates with Myc.
- p14ARF relocates Myc from the nucleoplasm to the nucleolus.
- p14ARF interacts with the Myc-Max complex via Myc BoxII.
- p14ARF downregulates Myc-activated transcription.
Conclusions:
- Human p14ARF directly binds and regulates Myc.
- The Myc BoxII domain is critical for p14ARF interaction.
- p14ARF inhibits Myc transcriptional activity through a non-repressor domain mechanism.
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