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Expression of matrix metalloproteinase-9 and -2 in pediatric chronic otitis media with effusion
Chul-Ho Jang1, Sung-Heui Shin, Hyong-Ho Cho
1Department of Otolaryngology, Chonnam National University Medical School, Hak-dong 8, Gwangju, South Korea. chulsavio@hanmail.net
Objective:
To evaluate the role of MMP-9 and MMP-2 in pediatric patients with middle ear effusion (MEE) and determine the pathogenesis of otitis media with effusion (OME) and allergy.
Methods:
The MEE samples were collected from 25 patients with allergy and 20 patients without allergy as a control. The levels of MMP-9 and MMP-2 were measured using a commercially available enzyme-linked immunosorbent assay (ELISA). The activity of MMP-9 and MMP-2 were measured by gelatin-zymography. The Mann-Whitney U-test was used for comparisons between the allergy positive and control groups.
Results:
The level of MMP-9 was significantly elevated in MEE from the allergy positive group compared to controls. The amount of MMP-9 activity significantly increased in the allergy positive group compared to controls.
Conclusion:
MMP-9 and MMP-2 are mediators of inflammation in the OME; in addition, MMP-9 may play a significant role in the pathophysiology of OME with allergy.
