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Updated: Aug 13, 2026
Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases
Published on: March 11, 2016
[Fundus autofluorescence examination using a confocal scanning laser ophthalmoscope HRA (Heidelberg Retina
Joanna Dolar-Szczasny1, Jerzy Mackiewicz, Almut Bindewald
1Z Katedry i I Kliniki Okulistyki Akademii Medycznej w Lublinie.
Purpose:
To present application of a confocal Scanning Laser Ophthalmoscope (cSLO) for fundus autofluorescence examination, as a new method of visualization of retinal pigment epithelium and its possible significance in the diagnosis of different retinal diseases.
Material And Methods:
Typical autofluorescence images in age-related macular degeneration (AMD), Stargardt disease, Best disease and pattern dystrophies are presented, based on the own experience and literature data. Autofluorescence images were obtained with a cSLO using an argon laser for generation of excitation light at 488 nm and a barrier filter >500 nm for the detection of the emitted signals.
Results:
A variety of autofluorescence patterns, associated with the accumulation of lipofuscin in RPE cells, was found in the above entities.
Conclusions:
Presented method of fundus autofluorescence examination gives new possibilities in studying the pathogenetic mechanisms in various retinal diseases and may be useful in monitoring the follow-up and the effects of the treatment.
