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Related Experiment Videos

Collagen and elastin gene expression in aortic aneurysms.

C L Mesh1, B T Baxter, W H Pearce

  • 1Department of Surgery, Northwestern University School of Medicine, Chicago, IL 60611.

Surgery
|August 1, 1992
PubMed
Summary
This summary is machine-generated.

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Abdominal aortic aneurysms (AAAs) show significantly increased type I collagen (COL I) gene expression but not elastin gene expression, explaining lower elastin levels. This enhanced COL I expression in AAAs is independent of age.

Area of Science:

  • Vascular Biology
  • Connective Tissue Research
  • Molecular Genetics

Background:

  • Abdominal aortic aneurysms (AAAs) are characterized by decreased elastin concentration.
  • This may stem from a differential gene expression where elastin fails to increase alongside type I procollagen (COL I).

Purpose of the Study:

  • To compare tissue messenger RNA (mRNA) levels of elastin and COL I in AAAs versus normal aortas.
  • To investigate the relationship between aging and COL I gene expression in aortic tissue.

Main Methods:

  • Northern analysis was performed on RNA extracted from normal (n=7) and AAA (n=10) aortic tissues.
  • Messenger RNA levels for COL I, elastin, and alpha-tubulin were quantified and compared using Student's t-test.
  • Regression analysis examined the correlation between age and COL I mRNA levels.

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Main Results:

  • COL I mRNA levels were significantly elevated in AAAs (1.18 ± 0.13) compared to normal aortas (0.14 ± 0.05).
  • Elastin mRNA levels did not show a commensurate increase in AAAs (0.11 ± 0.02) compared to normal aortas (0.39 ± 0.2).
  • No significant correlation was found between age and COL I gene expression.

Conclusions:

  • The relative decrease in elastin compared to collagen in AAAs can be partly attributed to altered elastin and collagen mRNA levels.
  • Enhanced COL I gene expression in AAAs is not associated with the patient's age.