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Related Experiment Videos

Microbial adhesion in flow displacement systems.

Henk J Busscher1, Henny C van der Mei

  • 1Department of Biomedical Engineering, University Medical Center Groningen and University of Groningen, P.O. Box 196, 9700 AD Groningen, The Netherlands.

Clinical Microbiology Reviews
|January 19, 2006
PubMed
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Flow displacement systems offer superior control over microbial adhesion studies compared to static methods. This review details their design and data analysis for biomedical applications.

Area of Science:

  • Microbiology
  • Biomedical Engineering
  • Surface Science

Background:

  • Static systems for microbial adhesion lack control over mass transport and shear conditions.
  • Rinsing steps in static systems to remove loosely adhering organisms are often ill-defined.
  • Flow displacement systems provide superior control over experimental parameters.

Purpose of the Study:

  • To review the fundamental principles of flow displacement systems for microbial adhesion research.
  • To provide guidance on calculating mass transport and shear rates.
  • To discuss system design, data analysis, and applications.

Main Methods:

  • Focus on the parallel plate flow chamber as a model system.
  • Explanation of calculations for mass transport and shear rates.

Related Experiment Videos

  • Discussion of critical design features and data analysis strategies.

    • Main Results:

    • Flow displacement systems enable precise control over mass transport and shear conditions.
    • Eliminates the need for ambiguous rinsing steps.
    • Facilitates reliable quantification of microbial adhesion.

    Conclusions:

    • Flow displacement systems are advantageous for studying microbial adhesion to surfaces.
    • The review provides a framework for utilizing these systems in research.
    • Applications span diverse biomedical fields, enhancing experimental rigor.