Related Experiment Video
Updated: Aug 13, 2026
Application of Microwave Ablation in Laparoscopic Partial Splenectomy
Published on: November 15, 2024
[Phagocytic function of macrophage after partial splenectomy of rabbits using microwave tissue coagulation]
Xinhua Ju1, Zhenlong Xia, Zhizhang Wang
1Department of General Surgery, The Second Affiliated Hospital of China Medical University, Shenyang 110004, China. juxinhuae@163.com
Abstract:
The immune function of spleen after partial splenectomy (PSM) of rabbits was stuided using microwave tissue coagulation (MTC). Streptococcus pneumoniae suspension was injected into the ear veins of rabbits 4 weeks after they were subjected to partial splenectomy using microwave tissue coagulation (PSM) group and to sham operation (SO groups), respectively. India ink was given via portal vein. Residual spleen were resected and microscopic examinations were performed on spleen slices stained using HE to compare phagocytic functions of macrophage. Gradings of phagocytic function in macrophage were of no significant difference between PSM and SO groups (P > 0.05). Weights of spleens showed no significant difference between the two groups (P > 0.05). The phagocytic function of macrophage after PSM can be preserved well. The result of this experiment implies that splenic salvage using MTC is a clinically applicable method.
Insights
Partial splenectomy using microwave tissue coagulation preserves spleen macrophage phagocytic function in rabbits. This splenic salvage method shows clinical applicability for maintaining immune function.
Area of Science:
- Immunology
- Surgical Pathology
- Veterinary Medicine
Context:
- Assessing spleen immune function post-partial splenectomy (PSM).
- Utilizing microwave tissue coagulation (MTC) for PSM in rabbits.
- Investigating macrophage phagocytic capacity as a key immune marker.
Purpose:
- To evaluate the impact of MTC-induced PSM on rabbit spleen immune function.
- To compare the phagocytic activity of splenic macrophages between PSM and sham operation (SO) groups.
- To determine the clinical applicability of MTC for splenic salvage.
Summary:
- Rabbits underwent PSM via MTC or SO, followed by Streptococcus pneumoniae and India ink challenge.
- Microscopic examination of residual spleen tissue revealed no significant difference in macrophage phagocytic function between PSM and SO groups (P > 0.05).
- Spleen weights also showed no significant difference, indicating preserved splenic function after MTC-induced PSM.
Impact:
- Demonstrates that MTC-induced PSM effectively preserves splenic macrophage phagocytic function.
- Suggests MTC is a viable clinical method for splenic salvage, maintaining crucial immune roles.
- Provides evidence supporting spleen-preserving surgical techniques in relevant medical fields.
