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Correlation between the anti-virus-induced cytopathic effect activity of interferon-alpha subtypes and induction of

Cécile Schanen1, Vincent Chieux, Pierre-Emmanuel Lobert

  • 1Service de Virologie/UPRES EA 3610 Faculté de Médecine, Université Lille 2, Centre Hospitalier Régional et Universitaire de Lille France, France.

Microbiology and Immunology
|January 24, 2006
PubMed

Insights

Different interferon-alpha (IFN-alpha) subtypes show varying biological effects due to their MxA protein induction capabilities. This study links IFN-alpha subtype differences in antiviral activity to their MxA protein induction in WISH cells.

Area of Science:

  • Immunology
  • Virology
  • Molecular Biology

Background:

  • Interferon-alpha (IFN-alpha) comprises multiple subtypes with unexplained variations in biological effects.
  • IFN-alpha's antiviral activity is partly mediated by inducing intracellular antiviral proteins.

Purpose of the Study:

  • To investigate if differences in biological effects among IFN-alpha subtypes correlate with their ability to induce antiviral proteins.
  • To examine the MxA protein induction and antiviral cytopathic effect (CPE) activity of 11 IFN-alpha subtypes.

Main Methods:

  • Human amnion WISH cells were treated with 11 IFN-alpha subtypes.
  • MxA protein levels were quantified using immunochemiluminescence assay.
  • Antiviral activity was assessed by protection against vesicular stomatitis virus (VSV)-induced CPE.

Main Results:

  • A wide range of MxA protein induction was observed across IFN-alpha subtypes at 10 and 100 IU/ml.
  • MxA protein levels correlated with anti-VSV-induced CPE at 10 IU/ml.
  • Significant disparities in MxA-inducing activity among IFN-alpha subtypes were identified.

Conclusions:

  • Differences in the anti-VSV-induced CPE activity of IFN-alpha subtypes in WISH cells are likely related to their varying capacities for MxA protein induction.
  • MxA protein induction serves as a potential indicator for the differential biological effects of IFN-alpha subtypes.

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