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Updated: Aug 13, 2026

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Published on: November 1, 2014
Equine CCL11 induces eosinophil cytoskeletal reorganization and activation
M C Weston1, M E Collins, F M Cunningham
1Department of Veterinary Basic Sciences, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hertfordshire AL9 7TA, UK. mweston@rvc.ac.uk
Objectives:
To assess the biological effects of purified recombinant equine CCL11 on equine eosinophil function.
Methods:
Following stimulation of eosinophils from normal horses, the polymerised form of actin was measured by flow cytometry using fluorescently labelled phalloidin. Migration was determined in a 96 well plate chemotaxis assay using 8 microm pore membranes, and adherence of eosinophils to serum-coated plastic was assessed using a colorimetric assay for eosinophil peroxidase. Superoxide generation was measured by the reduction of cytochrome C in a colorimetric assay.
Results:
Equine CCL11 induced significant (p < 0.001), concentration-dependent actin polymerisation and migration of equine eosinophils. Stimulation with CCL11 did not induce significant adherence to serum coated plastic, or superoxide production.
Conclusions:
Equine CCL11 stimulates cytoskeletal reorganization and migration of equine eosinophils, suggesting that it may be involved in the regulation of eosinophil trafficking in horses.
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