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Published on: April 13, 2022
[Immortalized human odontoblast-like cell line expressed dentin extracellular matrix in vitro]
Han-guo Wang1, Ming-zhen Xiao, Shou-liang Zhao
1Dept of Operative Dentistry and Endodontics, College of Stomatology, The Fourth Military Medical University, Xi'an, China.
Objective:
To determine whether dentin matrix proteins were expressed by the human odontoblast-like cell line hTERT-hOd-1 in vitro.
Methods:
Collagen type I, bone sialoprotein (BSP), dentin matrix protein 1 (DMP1) and the marker for odontoblast, dentin sialophosphoprotein (DSPP) and dentin sialoprotein (DSP) were detected in these cells by immunohistochemistry, RT-PCR and in situ hybridization. During being cultured in mineralizing medium for 5 weeks, the secretion of OC and activity of ALP were measured once a week.
Results:
DSPP, DMP1, BSP and collagen type I were expressed in hTERT-hOd-1 either at mRNA or protein level. Under the induction of mineralizing medium, the cells showed higher activity of ALP and increased secretion of OC.
Conclusion:
hTERT-hOd-1 expressed dentin extracellular matrix in vitro, which means the cell line has the potential of mineralization.
