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Related Experiment Videos

The E-Test and Campylobacter jejuni.

C N Baker1

  • 1Centers for Disease Control, Atlanta, Georgia 30333.

Diagnostic Microbiology and Infectious Disease
|July 1, 1992
PubMed
Summary
This summary is machine-generated.

The E-Test shows high agreement for antimicrobial susceptibility testing of Campylobacter jejuni. Adjusting breakpoints is needed for accurate erythromycin interpretation, favoring E-Test or agar dilution methods.

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Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Antimicrobial Resistance

Background:

  • Antimicrobial susceptibility testing (AST) is crucial for guiding treatment decisions.
  • Campylobacter jejuni is a significant cause of bacterial gastroenteritis.
  • Accurate AST methods are essential for monitoring and combating antimicrobial resistance.

Purpose of the Study:

  • To compare the E-Test with broth microdilution and agar dilution methods for AST of Campylobacter jejuni.
  • To evaluate the agreement between different AST methods.
  • To assess the impact of current breakpoints on the interpretation of erythromycin susceptibility.

Main Methods:

  • The E-Test, broth microdilution, and agar dilution methods were used.
  • Antimicrobial susceptibility was tested against 55 and 30 clinical isolates of Campylobacter jejuni.

Related Experiment Videos

  • Five antimicrobial agents, including erythromycin, tetracycline, and ciprofloxacin, were evaluated.

    • Main Results:

    • The E-Test demonstrated the best overall agreement (85%) with other methods within +/- 1 log2 dilution.
    • Category interpretation of erythromycin results posed challenges with current NCCLS breakpoints.
    • A significant proportion of strains were categorized as intermediate for erythromycin by E-Test and agar dilution (82%).

    Conclusions:

    • The E-Test is a reliable method for Campylobacter jejuni AST, showing good agreement with established techniques.
    • Current breakpoints for erythromycin may require adjustment to improve interpretation accuracy.
    • E-Test or agar dilution at 42°C for 16 hours are preferred methods for C. jejuni AST.