Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Silica microspheres for biomolecular detection applications.

A Demir1, A Serpengüzel

  • 1Department of Physics, Koç University, Sariyer, Istanbul, 34450, Turkey.

IEE Proceedings. Nanobiotechnology
|January 31, 2006
PubMed
Summary

Microsphere biosensors offer high sensitivity for biomolecule detection by monitoring shifts in light scattering. Silica microspheres are ideal photonic materials for this sensitive single-molecule detection technology.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Predictive Value of Early β-hCG Levels After Embryo Transfer in In Vitro Fertilization Treatment.

Nigerian journal of clinical practice·2026
Same author

The effect of the COVID-19 pandemic lockdown on symptom severity in school children with house dust mite-sensitized allergic rhinitis.

Revue francaise d'allergologie (2009)·2022
Same author

The effects of total intravenous and inhalation anesthesia maintenance on tissue oxygenation in coronary artery bypass graft surgery.

European review for medical and pharmacological sciences·2022
Same author

Incidence, detection and outcome of differentiated thyroid cancer in Western Sweden.

BJS open·2021
Same author

Comparison of inflammation markers with prediction scores in patients with community-acquired pneumonia.

Bratislavske lekarske listy·2021
Same author

Surgical treatment of nictitans gland prolapse and cartilage eversion accompanying the nictitating membrane (third eyelid) rotation in cats.

Polish journal of veterinary sciences·2021

Area of Science:

  • Photonics
  • Biotechnology
  • Materials Science

Background:

  • Microsphere-based biosensors are gaining attention for their sensitivity and selectivity.
  • Microspheres exhibit high quality-factor (Q-factor) morphology-dependent resonances sensitive to refractive index and size.
  • These resonances can be perturbed for biomolecule detection.

Purpose of the Study:

  • To investigate the use of microsphere morphology-dependent resonances for biosensing.
  • To analyze the sensitivity of silica microspheres for detecting biomolecular adsorption.
  • To evaluate scattering spectra for biosensor monitoring.

Main Methods:

  • Calculated transverse electric and transverse magnetic elastic scattering spectra at 0 and 90 degrees for silica microspheres at 1.55 micrometers.
  • Simulated shifts in resonance wavelengths due to the adsorption of a layer (representing biomolecules).
  • Utilized 90-degree scattering to monitor the scattered signal and 0-degree scattering for transmission signal.

Main Results:

  • Adsorption of biomolecules on microspheres changes effective size and refractive index, causing resonance wavelength shifts.
  • Silica, with a refractive index close to biomolecular agents, is a suitable material.
  • Calculations show resonance shifts after adding a layer, demonstrating the sensing principle.

Conclusions:

  • Microsphere-based biosensors can achieve highly sensitive, single-molecule detection.
  • The perturbation of morphology-dependent resonances is a viable mechanism for biosensing.
  • Silica microspheres are promising photonic materials for advanced biosensor development.

Related Experiment Videos