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Published on: August 19, 2016

Microinflammation in hemodialysis is related to a preactivated subset of monocytes

Rafael Ramirez1, Julia Carracedo, Isabel Berdud

  • 1Unidad de Investigación, Servicio de Nefrología, Hospital Universitario Reina Sofía, Córdoba, Spain. manuelr.ramirez.sspa@juntadeadalucia.es

Hemodialysis International. International Symposium on Home Hemodialysis
|January 31, 2006
PubMed

Insights

In hemodialysis patients, a specific monocyte subset (CD14+/CD16+) shows heightened inflammatory activity and phagocytosis. These primed cells exhibit proinflammatory characteristics, similar to those seen in Systemic Lupus Erythematosus and HIV.

Area of Science:

  • Immunology
  • Cell Biology
  • Nephrology

Background:

  • Monocytes with low CD14 and co-expressing CD16 (CD14+/CD16+) are increased in hemodialysis (HD) patients.
  • The functional significance of this monocyte subset in HD patients remains unclear.

Purpose of the Study:

  • To investigate if CD14+/CD16+ monocytes in HD patients are primed for proinflammatory activity.
  • To compare the immune function of CD14+/CD16+ monocytes with CD14++ monocytes in HD patients and other conditions.

Main Methods:

  • Flow cytometry was used to analyze CD14/CD16 expression on monocytes.
  • Immune function was assessed by measuring cytokine, chemokine, and sialoadhesin expression, as well as phagocytosis.
  • Cells from 32 HD patients, 9 Systemic Lupus Erythematosus (SLE) patients, 9 HIV-1 patients, and 15 healthy controls were analyzed.

Main Results:

  • HD patients exhibited an increased percentage of CD14+/CD16+ monocytes.
  • Compared to CD14++ monocytes, CD14+/CD16+ monocytes showed increased expression of proinflammatory cytokines and differentiated cell markers.
  • CD14+/CD16+ monocytes from HD patients demonstrated enhanced phagocytic activity, mirroring findings in SLE and HIV patients.

Conclusions:

  • CD14+/CD16+ monocytes in HD patients display characteristics of primed, prestimulated proinflammatory cells.
  • These findings suggest a potential role for this monocyte subset in the inflammatory state associated with hemodialysis.
  • The observed inflammatory profile of CD14+/CD16+ monocytes in HD is comparable to that in SLE and HIV patients.

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