Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure-based drug design meets the ribosome.

François Franceschi1, Erin M Duffy

  • 1Rib-X Pharmaceuticals, Inc., 300 George Street, Suite 301, New Haven, CT 06511, USA. ffranceschi@rib-x.com

Biochemical Pharmacology
|January 31, 2006
PubMed
Summary

High-resolution bacterial ribosome structures reveal antibiotic binding sites and resistance mechanisms. This knowledge enables the rational design of novel antibacterial drugs targeting RNA.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Considerations in Designing Clinical Trials of Novel Antibacterial Agents Using a Superiority Design.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2026
Same author

[Innovative medical devices in France: A descriptive study based on public healthcare databases].

Annales pharmaceutiques francaises·2025
Same author

Infection Risk Associated With Colonization by Multidrug-Resistant Gram-Negative Bacteria: An Umbrella Review and Meta-analysis.

Open forum infectious diseases·2025
Same author

Carbapenem-resistant Enterobacterales among patients with bloodstream infections in South Africa: Consolidated surveillance data, 2015-2021.

PloS one·2025
Same author

Quantitative performance of humanized plasma and epithelial lining fluid exposures of meropenem, cefiderocol and tobramycin against a challenge set of Klebsiella pneumoniae and Pseudomonas aeruginosa in a standardized neutropenic murine pneumonia model.

The Journal of antimicrobial chemotherapy·2025
Same author

[Development of a cost-satisfaction indicator for monitoring implantable medical devices on loan and in permanent storage].

Annales pharmaceutiques francaises·2024

Area of Science:

  • Structural Biology
  • Computational Chemistry
  • Drug Discovery

Background:

  • High-resolution structures of bacterial ribosomal subunits and their antibiotic complexes have significantly advanced understanding of small-molecule RNA interactions.
  • Abundant RNA structural data facilitates a drug discovery paradigm focused on RNA-based targets.

Purpose of the Study:

  • To elucidate antibiotic binding sites and mechanisms of action on bacterial ribosomes at the atomic level.
  • To understand how target-based resistance affects antibiotics acting on the ribosome.

Main Methods:

  • Analysis of high-resolution structures of bacterial ribosomal subunits (30S and 50S) and their complexes with antibiotics.
  • Utilizing a data-driven computational toolkit to predict molecular properties for drug design.

Main Results:

  • Identified specific sites where different antibiotic classes inhibit protein synthesis.
  • Revealed antibiotic orientations, relative dispositions, and unique mechanisms of action.
  • Characterized "targets of targets" with multiple antibiotic binding pockets and mapped resistance hotspots.

Conclusions:

  • Detailed knowledge of ribofunctional loci and resistance hotspots enables rational design of next-generation antibacterials.
  • Structure-based drug design, combined with computational tools for predicting cell penetration and drug-likeness, shows significant promise for novel antibacterial development.

Related Experiment Videos