Related Experiment Video
Updated: Aug 13, 2026
Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
Published on: June 28, 2024
New double quantum coherence filter for localized detection of glutathione in vivo
Tiejun Zhao1, Keith Heberlein, Carolyn Jonas
1The Wallace H. Coulter Department of Biomedical Engineering, Emory University/Georgia Institute of Technology, Atlanta GA, USA.
Abstract:
In this work, a new double quantum filter for glutathione (GSH) editing is introduced, combined with the point-resolved spectroscopy localization sequence (PRESS), and demonstrated in vivo. Compared to the conventional double quantum coherence filter, the new filter has two major advantages. First, it eliminates the need for calibration scans for optimizing the signal yield, making it more efficient and convenient for routine use. Second, it removes the influence of water saturation pulses on the GSH yield, further improving its accuracy. With this method, GSH concentrations in the left and right parietal lobes of five healthy volunteers were determined to be 0.91+/-0.16 mM and 0.89+/-0.16 mM, respectively, in agreement with previous studies.
