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Updated: Aug 13, 2026
High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
Published on: August 3, 2011
Parallel picoliter rt-PCR assays using microfluidics
Joshua S Marcus1, W French Anderson, Stephen R Quake
1Option in Biochemistry and Molecular Biophysics, Department of Applied Physics, California Institute of Technology, Pasadena, 91125, USA.
Abstract:
The development of microfluidic tools for high-throughput nucleic acid analysis has become a burgeoning area of research in the post-genome era. Here, we have developed a microfluidic chip to perform 72 parallel 450-pL RT-PCRs. We took advantage of Taqman hydrolysis probe chemistry to detect RNA templates as low as 34 copies. The device and method presented here may enable highly parallel single cell gene expression analysis.
