Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative comparison of the HSV-1 and HSV-2 transcriptomes using DNA microarray analysis.

J S Aguilar1, G V Devi-Rao, M K Rice

  • 1Department of Molecular Biology and Biochemistry and Center for Virus Research, University of California, Irvine, Irvine, CA 92697, USA. jsaguila@uci.edu

Virology
|February 2, 2006
PubMed
Summary

Human herpes virus type-1 and type-2 (HSV-1 and HSV-2) transcriptomes show distinct temporal accumulation patterns for four key genes involved in nuclear organization, potentially explaining their differing pathologies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Response to correspondence from McCarthy et al. regarding maternal sepsis screening and the role of the neutrophil-to-lymphocyte ratio.

International journal of obstetric anesthesia·2025
Same author

Incidence and sociodemographic, living environment and maternal health associations with stillbirth in a tertiary healthcare setting in Kano, Northern Nigeria.

BMC pregnancy and childbirth·2022
Same author

A qualitative focus group study concerning perceptions and experiences of Nigerian mothers on stillbirths.

BMC pregnancy and childbirth·2021
Same author

Rising trend of substance abuse in Pakistan: a study of sociodemographic profiles of patients admitted to rehabilitation centres.

Public health·2019
Same author

Standing intraoral extractions of cheek teeth aided by partial crown removal in 165 horses (2010-2016).

Equine veterinary journal·2017
Same author

Draft Genome Sequences of Six Different Staphylococcus epidermidis Clones, Isolated Individually from Preterm Neonates Presenting with Sepsis at Edinburgh's Royal Infirmary.

Genome announcements·2015

Area of Science:

  • Virology
  • Molecular Biology
  • Genomics

Background:

  • Human herpes virus type-1 (HSV-1) and type-2 (HSV-2) share high genomic sequence identity.
  • Despite sequence similarity, HSV-1 and HSV-2 exhibit significant differences in pathology.
  • Understanding transcriptional differences is crucial for explaining these pathological variations.

Purpose of the Study:

  • To comparatively analyze the temporal and abundance profiles of HSV-1 and HSV-2 transcriptomes.
  • To identify differences in gene expression patterns between the two virus types.
  • To investigate the potential link between transcriptional differences and pathological variations.

Main Methods:

  • Parallel infections of cell cultures with HSV-1 and HSV-2.
  • Microarray analysis using HSV-1 probes for cross-hybridization with HSV-2 transcripts.

Related Experiment Videos

  • Comparative analysis of transcript accumulation kinetics and relative abundance.

    • Main Results:

    • Orthologous transcripts from both HSV-1 and HSV-2 belong to the same kinetic expression class.
    • Significant differences were observed in the temporal accumulation patterns of four specific transcripts (U(L)4, U(L)29, U(L)30, and U(L)31).
    • The protein products of these differentially expressed transcripts are involved in nuclear organization and viral DNA localization.

    Conclusions:

    • Specific temporal differences in the transcription of HSV-1 and HSV-2 genes related to nuclear organization exist.
    • These transcriptional variations may contribute to the distinct pathological outcomes observed between HSV-1 and HSV-2 infections.
    • Further research is warranted to elucidate the precise role of these genes in viral pathogenesis.