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Pediococcus stilesii sp. nov., isolated from maize grains.
Charles M A P Franz1, Marc Vancanneyt2, Katrien Vandemeulebroecke2
1Federal Research Centre for Nutrition and Food, Institute for Hygiene and Toxicology, Haid-und-Neu-Strasse 9, D-76131 Karlsruhe, Germany.
International Journal of Systematic and Evolutionary Microbiology
|February 2, 2006
Summary
A novel lactic acid bacterium, Pediococcus stilesii, was identified from maize. This unique microbe grows at pH 9.0, distinguishing it from other Pediococcus species.
Area of Science:
- Microbiology
- Bacteriology
- Food Science
Background:
- Lactic acid bacteria (LAB) are crucial in food fermentation and gut health.
- The genus Pediococcus comprises several species with varying industrial and probiotic applications.
- Characterization of novel LAB strains is essential for expanding biotechnological potential.
Purpose of the Study:
- To isolate and characterize a novel lactic acid bacterium from steeped maize grains.
- To determine the phylogenetic and genotypic relationship of the isolate to known Pediococcus species.
- To identify unique physiological characteristics that differentiate the novel strain.
Main Methods:
- Isolation of bacteria from steeped maize grains.
- 16S rRNA gene sequencing for phylogenetic analysis.
- Genotypic fingerprinting, whole-cell protein electrophoresis, and DNA-DNA hybridization.
- Physiological and biochemical tests, including growth at different pH levels.
Main Results:
- A Gram-positive, coccus-shaped, homofermentative lactic acid bacterium, strain LMG 23082T, was isolated.
- Phylogenetic analysis placed the strain within the genus Pediococcus, closely related to Pediococcus pentosaceus and Pediococcus acidilactici.
- Strain LMG 23082T could be differentiated from other Pediococcus species using genotypic and phenotypic methods.
- A unique characteristic was the strain's ability to grow at pH 9.0, unlike other known pediococci.
Conclusions:
- Strain LMG 23082T represents a novel species, Pediococcus stilesii sp. nov.
- Pediococcus stilesii is distinguished by its ability to grow at alkaline pH.
- This discovery expands the known diversity within the Pediococcus genus and offers potential for new applications.