Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

1.8K
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
1.8K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.9K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The study of distribution and fate of nitrobenzene in a water/sediment microcosm.

Chemosphere·2007
Same author

In vivo dedifferentiation of human epidermal cells.

Cell biology international·2007
Same author

What is in a word? No versus Yes differentially engage the lateral orbitofrontal cortex.

Emotion (Washington, D.C.)·2007
Same author

[Gene expression profile changes in oral verrucous carcinoma and oral squamous cell carcinoma].

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2007
Same author

Morphology of critical nuclei in solid-state phase transformations.

Physical review letters·2007
Same author

Enhanced cooperative activation effect in the hydrolytic kinetic resolution of epoxides on [Co(salen)] catalysts confined in nanocages.

Angewandte Chemie (International ed. in English)·2007

Related Experiment Video

Updated: Apr 29, 2026

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

17.0K

Negative pressure pinched sample injection for microchip-based electrophoresis.

Lei Zhang1, Xuefeng Yin, Zhaolun Fang

  • 1Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, 310027, Hangzhou, China.

Lab on a Chip
|February 2, 2006
PubMed
Summary

A new method simplifies sample injection for microfluidic capillary electrophoresis (CE) using negative pressure and electrokinetic forces. This technique improves throughput and precision for analyzing samples like rhodamine123 and fluorescein sodium.

More Related Videos

Picoinjection of Microfluidic Drops Without Metal Electrodes
09:20

Picoinjection of Microfluidic Drops Without Metal Electrodes

Published on: April 18, 2014

10.7K
Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
14:12

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

2.9K

Related Experiment Videos

Last Updated: Apr 29, 2026

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

17.0K
Picoinjection of Microfluidic Drops Without Metal Electrodes
09:20

Picoinjection of Microfluidic Drops Without Metal Electrodes

Published on: April 18, 2014

10.7K
Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
14:12

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

2.9K

Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Separation Science

Background:

  • Microfluidic chip-based capillary electrophoresis (CE) systems require precise sample injection for accurate separations.
  • Traditional pinched injection methods can be complex, requiring specialized equipment and multiple pumps.

Purpose of the Study:

  • To develop a simplified method for injecting well-defined, non-biased sample plugs into microfluidic CE systems.
  • To reduce the complexity of equipment and operations for pinched injection in chip-based CE.

Main Methods:

  • A novel approach combining negative pressure, electrokinetic, and hydrostatic forces for sample plug formation.
  • Utilized a single syringe pump and a single voltage supply for both sample loading and electrokinetic injection.
  • Employed a vacuum in the sample waste reservoir headspace to draw sample and buffer flow to create the plug.

Main Results:

  • Achieved well-defined sample plug formation at the channel intersection.
  • Demonstrated successful electrokinetic injection into the separation channel after vacuum release.
  • Obtained high precision for migration times (1.5–3.3% RSD) and peak heights (4.4–6.2% RSD) for rhodamine123 and fluorescein sodium over 56 consecutive determinations.

Conclusions:

  • The developed method significantly simplifies operations and equipment for pinched injection in chip-based CE.
  • The approach enhances throughput and analytical precision for microfluidic CE applications.
  • This technique offers a robust and efficient solution for sample handling in miniaturized separation systems.