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Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
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Cloning and preliminary characterization of a GATC-specific beta2-class DNA:m6A methyltransferase encoded by

Monika Radlińska1, Andrzej Piekarowicz, Marc Galimand

  • 1Institute of Microbiology, University of Warsaw, ul. Miecznikowa 1, 02-096 Warszawa, Poland. m.radlinska@biol.uw.edu.pl

Polish Journal of Microbiology
|February 3, 2006
PubMed

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DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
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Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...