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Related Experiment Video

Updated: Dec 21, 2025

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Published on: January 31, 2025

Signal dynamics in Sonic hedgehog tissue patterning.

Krishanu Saha¹, David V Schaffer

¹Department of Chemical Engineering and the Helen Wills Neuroscience Institute, University of California, Berkeley, 94720-1462, USA.

Development (Cambridge, England)

|February 3, 2006

Summary

This summary is machine-generated.

This study models Sonic hedgehog (Shh) morphogen dynamics, revealing how transport mechanisms dynamically regulate neural tube patterning. Slowing Shh transport can enhance its signaling range, while certain targets limit it.

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Area of Science:

Developmental Biology
Systems Biology
Computational Biology

Background:

Morphogens are crucial for development, guiding cell differentiation via concentration gradients.
Understanding morphogen gradient formation is complex, often studied at steady state.

Purpose of the Study:

To develop a theoretical model analyzing the dynamic interplay of transport and signal transduction for morphogen patterning.
To investigate how Sonic hedgehog (Shh) gradient dynamics influence neural tube development.

Main Methods:

Developed a theoretical model for morphogen transport and signal transduction.
Analyzed the dynamic formation of morphogen gradients.

Main Results:

Model confirms that gradient formation dynamics are key determinants of cell response.
Slower Shh transport (e.g., via lipid modification, proteoglycan binding, Dispatched upregulation) can increase signaling range by concentrating the morphogen near the source.
Transcriptional targets like Patched and Hedgehog-interacting protein limit Shh signaling range by slowing transport and promoting degradation.

Conclusions:

Modular transport mechanisms dynamically shape tissue patterns during development.
Understanding these dynamic processes provides novel insights into morphogen-controlled patterning.