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Related Experiment Videos

Langerhans cells--revisiting the paradigm using genetically engineered mice.

Adrien Kissenpfennig1, Bernard Malissen

  • 1Centre d'Immunologie de Marseille-Luminy, INSERM-CNRS-Université de la Méditerrannée, Parc Scientifique et Technologique de Luminy, Case 906, 13288 Marseille Cedex 09, France.

Trends in Immunology
|February 7, 2006
PubMed
Summary

New knockin mice allow researchers to track Langerhans cells (LCs), a type of dendritic cell (DC), and study their function in skin immunity and tolerance. These models help define LC roles in immune responses.

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Corrigendum to "Repeat application of microneedles does not alter skin appearance or barrier function and causes no measurable disturbance of serum biomarkers of infection, inflammation or immunity in mice in vivo" [Eur. J. Pharm. Biopharm. 117 (2017) 400-407].

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Area of Science:

  • Immunology
  • Dendritic Cell Biology
  • Skin Immunity

Background:

  • Langerhans cells (LCs) are key dendritic cells (DCs) in epithelia.
  • Their precise roles in immunity and tolerance remain incompletely understood.
  • Distinguishing LCs from other DC subsets in vivo has been challenging.

Purpose of the Study:

  • To review insights gained from novel knockin mouse models for studying LCs.
  • To assess the utility of these models in tracking LC dynamics and function.
  • To identify knowledge gaps regarding LC biology and immune roles.

Main Methods:

  • Development of knockin mice expressing enhanced green fluorescent protein (EGFP) under the langerin (CD207) gene to identify and track epidermal LCs.
  • Utilized additional knockin mice expressing a diphtheria toxin receptor fused to EGFP for conditional ablation of LCs.

Related Experiment Videos

  • Employed skin immunization models to evaluate LC involvement in hapten-specific T cell responses.

    • Main Results:

    • EGFP-expressing knockin mice enable clear discrimination and in vivo tracking of epidermal LCs.
    • Conditional ablation models facilitate the assessment of LC-dependent immune responses.
    • These models provide valuable tools for dissecting LC contributions to skin immunity.

    Conclusions:

    • Knockin mouse models offer powerful approaches to investigate Langerhans cell biology.
    • Further research is needed to fully elucidate the complex roles of LCs in immunity and tolerance.
    • These models are crucial for advancing our understanding of epidermal immunology.