Immunohistochemical localization of transcription factor Sp3 during dental enamel development in rat tooth germ

Sp3, a member of the Sp family of transcription factors, has previously been thought to be ubiquitously expressed, and its expression pattern in tooth development is not clear. This study was carried out to investigate the immunolocalization of Sp3 during the development of rat tooth germs. Sprague-Dawley rats at ages of 1, 3, 7, 10, and 14 d were used to represent different stages of tooth development. First mandibular molar tooth germs were sectioned and studied by immunohistochemistry. Sp3 was found to be localized within the nuclei of cells in developing tooth germs; however, ameloblast nuclei showed variable intensities at different developmental stages. At the same time, the positive signals in odontoblast nuclei remained stable. The results suggest that Sp3 may play a role in the development of teeth, specifically in the transcription of enamel-specific genes.