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Characterization of an Indonesian isolate of Paecilomyces reniformis

O Kalkar1, G R Carner, D Scharf

  • 1Department of Biology, Kahramanmaras Sütçü Imam University, Kahramanmaraş, Turkey. ozlemk@ksu.edu.tr

Mycopathologia
|February 8, 2006
PubMed

Insights

A new entomopathogenic fungus, Paecilomyces reniformis (IndGH 96), was identified from Indonesian grasshoppers. This fungus infects grasshoppers but not other insects, offering potential for biological pest control.

Area of Science:

  • Mycology
  • Entomology
  • Biological Pest Control

Background:

  • Entomopathogenic fungi are crucial for regulating insect populations.
  • Paecilomyces species are known pathogens of various arthropods.
  • Isolation of novel fungal strains aids in developing sustainable pest management strategies.

Purpose of the Study:

  • To characterize a newly isolated entomopathogenic fungus, Paecilomyces reniformis (IndGH 96).
  • To determine the phylogenetic relationship of the isolate using molecular and morphological data.
  • To evaluate the pathogenicity of the isolate against different insect species.

Main Methods:

  • Morphological characterization of fungal growth and conidia.
  • DNA sequencing of ITS, 28S rDNA D1/D2, and tubulin gene regions.
  • Laboratory bioassays to assess pathogenicity against grasshoppers, crickets, and caterpillars.

Main Results:

  • The isolate IndGH 96 was confirmed as Paecilomyces reniformis based on phenotypic and molecular analyses.
  • Distinctive features include a hyphal body stage and curved, light-green conidia.
  • IndGH 96 demonstrated infectivity against long-horned and short-horned grasshoppers but not against crickets or lepidopterans.

Conclusions:

  • Paecilomyces reniformis (IndGH 96) is a grasshopper-specific entomopathogen.
  • Phylogenetic analysis suggests a closer relationship between P. reniformis and Nomuraea rileyi.
  • This finding contributes to understanding fungal diversity and developing targeted biocontrol agents.

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