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Updated: Aug 11, 2026

Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique
Published on: November 11, 2022
State-dependent block of human cardiac hNav1.5 sodium channels by propafenone
1Cellular Electrophysiology Laboratory, Department of Anesthesia, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA. tedrich@partners.org
Abstract:
State-dependent blockade of human cardiac hNav1.5 sodium channels by propafenone was studied using whole-cell patch clamp techniques. Both a direct investigation using cells with inactivation-deficient sodium channels and an algorithmic approach used on cells with wild-type channels revealed a rapid binding of propafenone to the open state. This occurs approximately 4000 and 700 times faster than the binding to the resting and inactivated state, respectively. An established mathematical "gating" model was modified to represent the experimental data.
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