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Phenotypic characterization of food waste degrading Bacillus strains isolated from aerobic bioreactors
Maria Teresa Saraiva Lopes da Silva1, Fátima Espírito Santo, Pablo Tavares Pereira
1Instituto Nacional de Engenharia, Tecnologia e Inovação, Departamento de Biotecnologia, Laboratório de Microbiologia Industrial, Estrada do Paço do Lumiar 22, 1649-038 Lisboa codex, Portugal.
Abstract:
A phenotypic characterization of seventeen Bacillus strains isolated from aerobic thermophilic bioreactors of a food waste processing company was carried out, using fatty acid and enzymatic activity profiles. It was observed that each species possessed a typical fatty acid and enzymatic production profile. Bacillus licheniformis strains exhibited the most significant enzyme production. Numerical analyses (principal component and hierarchical cluster analyses) revealed that Bacillus licheniformis strains were homogeneous regarding their fatty acid profiles whilst B. subtilis and Bacillus pumilus strains showed some phenotypic differences. However, enzymatic activities numerical analyses indicated that these three Bacillus species were more homogeneous regarding this phenotypic characteristic.
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