Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Direct assay for alpha-amylase using fluorophore-modified cyclodextrins.

Takuya Murayama1, Tetsuya Tanabe, Hiroshi Ikeda

  • 1Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B-44 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.

Bioorganic & Medicinal Chemistry
|February 9, 2006
PubMed
Summary

A new assay method uses fluorophore-modified cyclodextrins (CDs) to detect alpha-amylase. The most sensitive substrate, NC4gammaCD, shows a linear correlation with enzyme concentration for accurate alpha-amylase quantification.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

p-Type Organic Semiconductors With an Annulated Thiazole: Photochemical Synthesis and Theoretically High Hole Mobility Predicted From Crystal Structure.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Prediction of Human Serum Concentration-Time Profiles of Golimumab and Ustekinumab Using Pharmacokinetic Data From Common Marmosets With Assessment of Anti-Drug Antibodies.

Biopharmaceutics & drug disposition·2026
Same author

Effect of Alumina Airborne-Particle Abrasion Followed by Plasma Treatment on Bond Strength of Dental PEEK to MMA-Based Luting Systems.

Bioengineering (Basel, Switzerland)·2026
Same author

Phenotypic and genetic variation among Rhododendron arboreum species complex widely distributed throughout the Himalayas suggests an obscure geographic origin of the clade.

Annals of botany·2026
Same author

Therapeutic Effects of Capacitive and Resistive Monopolar Radiofrequency on Muscle Stiffness and Local Hemodynamics of the Rectus Femoris: A Randomized Crossover Study of Female University Soccer Players.

International journal of sports physical therapy·2026
Same author

A low-toxicity ammonium hydrogen fluoride-based etchant for bonding pretreatment of silica-based CAD-CAM materials.

Dental materials journal·2026

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Enzymology

Background:

  • Alpha-amylase is a crucial enzyme in carbohydrate metabolism.
  • Existing assay methods for alpha-amylase can be complex or lack sensitivity.
  • Cyclodextrins (CDs) are versatile hosts with potential for enzyme substrate development.

Purpose of the Study:

  • To develop a simple and sensitive assay method for alpha-amylase detection.
  • To design and synthesize novel fluorophore-modified cyclodextrin derivatives as artificial substrates.
  • To evaluate the performance of these substrates in alpha-amylase quantification.

Main Methods:

  • Synthesis of four NBD-amine modified cyclodextrin derivatives.
  • Assaying alpha-amylase activity using fluorescence intensity changes.

Related Experiment Videos

  • Determining the optimal substrate (NC4gammaCD) for alpha-amylase detection.
  • Correlating hydrolysis rates with enzyme concentration.

    • Main Results:

    • All NBD-amine modified CDs showed decreased fluorescence upon alpha-amylase addition.
    • NC4gammaCD exhibited the highest sensitivity as an alpha-amylase substrate.
    • A linear relationship was observed between NC4gammaCD hydrolysis rate and alpha-amylase concentration.
    • The NC4gammaCD substrate demonstrated specificity for alpha-amylase over other guest compounds.

    Conclusions:

    • Fluorophore-modified cyclodextrins provide a sensitive platform for alpha-amylase assays.
    • NC4gammaCD is a highly effective substrate for quantifying alpha-amylase.
    • This method offers a simple, sensitive, and specific approach for alpha-amylase detection.