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Related Experiment Videos

Decrease in membrane-associated actin of fibroblasts after transformation by Rous sarcoma virus.

G Wickus, E Gruenstein, P W Robbins

    Proceedings of the National Academy of Sciences of the United States of America
    |February 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

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    Rous sarcoma virus transformation reduces membrane-associated actin by 30-50% in fibroblasts. This selective displacement, not a general actin decrease, occurs at permissive temperatures.

    Area of Science:

    • Cell Biology
    • Virology
    • Biochemistry

    Background:

    • Actin is a crucial cytoskeletal protein involved in cell structure and motility.
    • Rous sarcoma virus (RSV) is a retrovirus known to induce cell transformation and alter cellular functions.
    • Membrane-associated proteins play key roles in cell signaling and adhesion.

    Purpose of the Study:

    • To quantify the changes in membrane-associated actin levels in cultured chick embryo fibroblasts after Rous sarcoma virus transformation.
    • To investigate whether the observed changes in actin are a result of general protein synthesis alterations or selective membrane displacement.
    • To determine the role of viral temperature-sensitive mutants in modulating membrane-associated actin.

    Main Methods:

    • Cultured chick embryo fibroblasts were used for the study.

    Related Experiment Videos

  • Polyacrylamide gel electrophoresis was employed to measure actin content in prepared membranes.
  • Tryptic peptide mapping was utilized for the identification of actin.
  • Experiments involved infection with Rous sarcoma virus and its temperature-sensitive strains.

    • Main Results:

    • A significant decrease (30-50%) in membrane-associated actin was observed after Rous sarcoma virus transformation.
    • Infection with a temperature-sensitive RSV strain reduced membrane-associated actin only at the permissive temperature (36°C).
    • Shifting to the permissive temperature increased the proportion of total cellular protein synthesis dedicated to actin production.

    Conclusions:

    • Rous sarcoma virus transformation leads to a selective displacement of actin from cell membranes.
    • The observed decrease in membrane-associated actin is not due to a general reduction in actin synthesis but rather a specific effect of viral transformation.
    • Temperature-dependent regulation by RSV influences the association of actin with cell membranes.