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Comparison of Two Enzyme-linked Immunosorbent Assays for Detecting Antibody to Rodent Viruses

M Middleton, W J Pouch, J Cope

    Contemporary Topics in Laboratory Animal Science
    |January 1, 1993
    PubMed
    Abstract

    No abstract available in PubMed .

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    Enzyme-Linked Immunosorbent Assay01:33

    Enzyme-Linked Immunosorbent Assay

    In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
    There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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