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Related Concept Videos

DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
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Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
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Related Experiment Video

Updated: Jul 24, 2026

Electroeluting DNA Fragments
06:13

Electroeluting DNA Fragments

Published on: September 6, 2010

Dielectrophoretic manipulation of DNA.

R Hölzel1, F F Bier

  • 1Fraunhofer Institut for Biomedical Engineering, Department of Molecular Bioanalytics and Bioelectronics, Bergholz-Regbrücke, Germany.

IEE Proceedings. Nanobiotechnology
|February 14, 2006
PubMed
Summary

AC electrokinetics, like dielectrophoresis, now enable precise spatial control of single DNA molecules. This review covers dielectrophoretic studies for genetic analysis and nanoscale construction.

Area of Science:

  • Biophysics
  • Nanotechnology
  • Molecular Biology

Background:

  • AC electrokinetic methods (dielectrophoresis, electrorotation) are established for cell manipulation.
  • Applications to submicroscopic entities like viruses and molecules are less common but increasing.
  • Advancements in electrode technology and single-molecule techniques drive this growth.

Purpose of the Study:

  • To review dielectrophoretic studies focused on single DNA molecules.
  • To highlight the potential of dielectrophoresis for genetic investigations.
  • To explore the use of dielectrophoresis in building nanostructures with high resolution.

Main Methods:

  • Review of existing literature on dielectrophoresis.
  • Focus on studies involving single-stranded and double-stranded DNA.

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Last Updated: Jul 24, 2026

Electroeluting DNA Fragments
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Published on: September 6, 2010

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

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DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

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  • Emphasis on single-molecule manipulation and characterization.

    • Main Results:

    • Dielectrophoresis offers precise spatial control of individual DNA molecules.
    • Demonstrated feasibility for genetic analysis and nanostructure assembly.
    • Emerging techniques enable manipulation at the nanoscale.

    Conclusions:

    • Dielectrophoresis is a powerful tool for single DNA molecule manipulation.
    • Significant potential for applications in genetic research and nanotechnology.
    • Continued development promises further advancements in molecular control.