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Related Experiment Videos

Shear stress facilitates tissue-engineered odontogenesis.

M J Honda1, Y Shinohara, Y Sumita

  • 1Tooth Regeneration, The Division of Stem Cell Engineering, The Institute of Medical Science, The University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. honda-m@ims.u-tokyo.ac.jp

Bone
|February 14, 2006
PubMed
Summary
This summary is machine-generated.

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Shear stress significantly enhances odontogenic cell differentiation and promotes tooth tissue engineering. This study shows shear stress application leads to enamel and dentin formation in vivo.

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Cell Biology

Background:

  • Osteoblast response to shear stress is documented, but its impact on odontogenic cells remains unexplored.
  • Odontogenesis, the process of tooth formation, involves complex cellular signaling and differentiation pathways.

Purpose of the Study:

  • To investigate the effect of shear stress on facilitating tissue-engineered odontogenesis using dissociated single cells.
  • To evaluate the potential of shear stress in promoting tooth regeneration and development.

Main Methods:

  • Harvesting porcine third molar cells at early crown formation stage.
  • Seeding heterogeneous cells on polyglycolic acid fiber mesh to create cell-polymer constructs.
  • Applying controlled shear stress to constructs and evaluating responses through in vitro and in vivo studies.

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Main Results:

  • Shear stress significantly enhanced odontogenic-related mRNA expression within 2 hours.
  • Amelogenin, bone sialoprotein, and vimentin protein expression increased significantly after 12 hours of shear stress.
  • Alkaline phosphatase activity increased significantly after 7 days without affecting cell proliferation.
  • In vivo implantation resulted in enamel and dentin formation in shear stress-exposed constructs after 15 weeks.

Conclusions:

  • Shear stress is a critical factor that facilitates odontogenic cell differentiation in vitro.
  • Applying shear stress is a promising strategy for advancing tooth tissue engineering and regeneration in vivo.