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Related Experiment Videos

The MCK enhancer contains a p53 responsive element.

H Weintraub1, S Hauschka, S J Tapscott

  • 1Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, WA 98104.

Proceedings of the National Academy of Sciences of the United States of America
|June 1, 1991
PubMed
Summary
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The tumor suppressor p53 activates the muscle-specific creatine kinase gene. This study identifies a novel p53 responsive element, advancing understanding of p53

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Regulation

Background:

  • The p53 protein is a critical tumor suppressor, frequently inactivated in human cancers.
  • The precise function of p53 in normal cellular processes remains largely unelucidated.

Purpose of the Study:

  • To investigate the role of p53 in regulating gene expression.
  • To identify and characterize a p53 responsive element within the muscle-specific creatine kinase gene.

Main Methods:

  • Co-transfection of mouse p53 with a reporter gene construct containing upstream sequences of the muscle-specific creatine kinase gene.
  • Analysis of reporter gene activation to determine p53-mediated transcriptional control.

Main Results:

  • Co-transfection resulted in a significant 10- to 80-fold activation of the reporter gene.

Related Experiment Videos

  • The identified p53 responsive element is distinct from the known MyoD binding site within the gene's regulatory region.

    • Conclusions:

    • p53 plays a role in activating the muscle-specific creatine kinase gene.
    • The discovery of a novel p53 responsive element facilitates focused research into p53's gene regulatory functions.