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Updated: Aug 11, 2026

Spatial Separation of Molecular Conformers and Clusters
Published on: January 9, 2014
Capillary zone electrophoretic chiral discrimination using 6-O-(2-hydroxy-3-trimethylammoniopropyl)-beta-cyclodextrin
Xiuli Lin1, Minggang Zhao, Xinyu Qi
1School of Pharmacy, Shandong University, Jinan, PR China.
Abstract:
A charged highly water-soluble CD derivative, 6-O-(2-hydroxy-3-trimethylammoniopropyl)-beta-CD (herein noted as 6-HPTMA-beta-CD) was synthesized and successfully used as a chiral selector for enantiomeric separation of some acidic compounds by CZE in an uncoated capillary. Substitution with 2-hydroxy-3-trimethylammoniopropyl groups at the primary hydroxyl group of the CD was aimed at influencing the magnitude and selectivity of analyte-CD interactions. The behavior of 6-HPTMA-beta-CD was compared with that of the commercially available quaternary ammonium-beta-CD (QA-beta-CD) under the same separating conditions. The experiments were carried out using a BGE consisting of 50 mM phosphate in the pH range of 4-6 by adding a relatively low concentration of chiral selector (less than 10 mM). The effects of the concentration of CD and the pH of the electrolyte on the resolution of these compounds were studied.
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