Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The structural basis for docking in modular polyketide biosynthesis.

Kira J Weissman1

  • 1Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK. kjw21@cus.cam.ac.uk

Chembiochem : a European Journal of Chemical Biology
|February 14, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeted Protein Degradation.

JACS Au·2026
Same author

Decision making by modular polyketide synthases and implications for genetic engineering.

Natural product reports·2026
Same author

Docking domains from modular polyketide synthases and their use in engineering.

Nature communications·2025
Same author

Protocol for the purification, analysis, and handling of acyl carrier proteins from type I fatty acid and polyketide synthases.

STAR protocols·2025
Same author

Correction: Exploiting the inherent promiscuity of the acyl transferase of the stambomycin polyketide synthase for the mutasynthesis of analogues.

Chemical science·2025
Same author

Exploiting the inherent promiscuity of the acyl transferase of the stambomycin polyketide synthase for the mutasynthesis of analogues.

Chemical science·2025
Same journal

Reaction Optimization for Enzymatic Deconstruction of Industrially Relevant Nylon Composites.

Chembiochem : a European journal of chemical biology·2026
Same journal

Deploying Artificial Metalloenzymes in Complex Environments: Strategies and Applications.

Chembiochem : a European journal of chemical biology·2026
Same journal

Synthetic Ligands of Myeloid C-Type Lectin Receptors.

Chembiochem : a European journal of chemical biology·2026
Same journal

Vancomycin-Mediated Binding of DNA Origami Nanostructures to Gram-Positive and Gram-Negative Bacteria.

Chembiochem : a European journal of chemical biology·2026
Same journal

Mutasynthesis and Antibiotic Activity of Mupirocin Analogues.

Chembiochem : a European journal of chemical biology·2026
Same journal

Pressure-Dependent Aromatic Ring Flips Reveal Variable Transition-State Volume and Compressibility Among Structural Regions of BPTI.

Chembiochem : a European journal of chemical biology·2026
See all related articles

Researchers genetically engineered polyketide synthase (PKS) docking domains, crucial for natural product biosynthesis. Helix swaps confirmed a four-alpha-helix bundle model, identifying key residues for controlling PKS subunit interactions.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Natural Product Biosynthesis

Background:

  • Polyketide synthases (PKSs) assemble natural products like erythromycin.
  • PKS subunits interact via docking domains, essential for accurate biosynthesis.
  • A four-alpha-helix bundle model has been proposed for PKS docking.

Purpose of the Study:

  • To genetically engineer PKS docking domains.
  • To test the proposed four-alpha-helix bundle model of PKS docking.
  • To identify residues influencing docking domain interactions.

Main Methods:

  • Genetic engineering of docking domain helical segments.
  • In vivo analysis of triketide synthesis using mutant PKSs.
  • Structural analysis of protein-protein interactions in PKS assembly.

Related Experiment Videos

Main Results:

  • Helix swaps in docking domains were successfully created.
  • Mutant PKSs produced triketides, validating the engineered domains.
  • Results support the four-alpha-helix bundle model for PKS docking.

Conclusions:

  • The four-alpha-helix bundle model accurately describes PKS docking.
  • Specific residues within docking domains can be targeted to modulate subunit interactions.
  • This work provides insights for engineering hybrid PKSs for novel compound synthesis.