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Updated: Aug 11, 2026

Establishing Cell Lines Overexpressing DR3 to Assess the Apoptotic Response to Anti-mitotic Therapeutics
Published on: January 11, 2019
[Experimental modulation of apoptosis: physiopathological and therapeutic targets]
Claudia Mitchell1, Vincent Mallet, Jacques-Emmanuel Guidotti
1Institut Cochin, Département de Génétique, Développement et Pathologie Moléculaires, U567 INSERM, CNRS 8101 UMR, Université R. Descartes, 24, rue du Faubourg Saint-Jacques, 75014 Paris, France.
Abstract:
In the liver, the importance of apoptosis is not only evident during development and homeostasis of the biliary tree but plays also a prominent role in liver pathogenesis. Ligand binding to cell surface death receptors such as Fas activates the extrinsic pathway. This pathway predominates in autoimmune liver diseases, viral hepatitis, liver allograft rejection. Hepatocyte apoptosis is also significantly increased in patients with alcoholic hepatitis and nonalcoholic steatohepatitis and correlates with disease severity and hepatic fibrosis. We have used this specific susceptibility of the liver to apoptosis to develop two different approaches: 1) a cell therapy strategy based on a survival advantage to an apoptotic stimulus conferred to transplanted hepatocytes and 2) a new model of hepatocyte conditional ablation based on a controlled activation of the cell death program.
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