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Related Experiment Videos

Electrostatic stitching in gel-phase supported phospholipid bilayers.

Liangfang Zhang1, Tighe A Spurlin, Andrew A Gewirth

  • 1Materials Research Laboratory, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.

The Journal of Physical Chemistry. B
|February 14, 2006
PubMed
Summary
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Adding cationic lipids to zwitterionic lipids creates defect-free gel-phase supported lipid bilayers. This method stabilizes bilayers, unlike anionic lipids or no charged lipids, by electrostatically "stitching" them together.

Area of Science:

  • Biophysics
  • Materials Science
  • Surface Chemistry

Background:

  • Supported lipid bilayers (SLBs) are crucial models for cell membranes.
  • Defects in SLBs can compromise their function and limit applications.
  • Controlling SLB morphology is essential for reliable experimental outcomes.

Purpose of the Study:

  • To investigate the effect of cationic lipids on the morphology of zwitterionic supported lipid bilayers.
  • To determine if cationic lipids can prevent defect formation in gel-phase SLBs.
  • To understand the mechanism behind defect stabilization.

Main Methods:

  • Preparation of supported lipid bilayers by vesicle fusion.
  • Incorporation of varying mole percentages of cationic lipids into zwitterionic lipid mixtures.

Related Experiment Videos

  • Morphological characterization using noncontact mode atomic force microscopy (AFM).
  • Infrared spectroscopy to analyze lipid headgroup orientation.

    • Main Results:

    • Mixing zwitterionic lipids with up to 20% cationic lipids resulted in defect-free gel-phase SLBs, as observed by AFM.
    • Anionic lipids or the absence of charged lipids led to significant bilayer defects.
    • Infrared measurements indicated minimal changes in headgroup orientation upon transitioning to the gel phase in the presence of cationic lipids.

    Conclusions:

    • Cationic lipids effectively stabilize zwitterionic lipid bilayers, preventing defect formation.
    • Electrostatic interactions, described as
    • stitching
    • by cationic lipids, are proposed as the mechanism for stabilization.
    • This study presents a simple and effective method for creating morphologically stable gel-phase supported lipid bilayers.