Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Force-controlled equilibria of specific vesicle-substrate adhesion.

Ana-Suncana Smith1, Barbara G Lorz, Stefanie Goennenwein

  • 1E22 Lehrstuhl für Biophysik, Technische Universität München, D-85748 Garching, Germany. asmith@ph.tum.de

Biophysical Journal
|February 14, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correlations in cadherin-mediated membrane adhesion dynamics: Molecular concentration switch and diffusion dial.

Biophysical journal·2026
Same author

Diversity in emergent cell locomotion from the coupling cytosolic and cortical Marangoni flows with reaction-diffusion dynamics.

PLoS computational biology·2026
Same author

Deciphering Ball Milling Mechanochemistry via Molecular Simulations of Collision-Driven and Liquid-Assisted Reactivity.

Angewandte Chemie (International ed. in English)·2025
Same author

Tiny Clusters for Big Impact in T Cell Activation.

Nano letters·2025
Same author

Shape-Selective Separation of Model Analytes in Normal-Phase Liquid Chromatography: A Combined Simulation-Experimental Study.

Analytical chemistry·2025
Same author

How the ionic liquid [C<sub>2</sub>C<sub>1</sub>Im][OTf] affects the stability of Pt(111) during potential cycling.

Physical chemistry chemical physics : PCCP·2025

New magnetic tweezers precisely measure pico-Newton forces on cell adhesion. Force-independent binding area decrease and shape analysis reveal bond numbers, advancing biophysics research.

Area of Science:

  • Biophysics
  • Cellular Mechanics
  • Molecular Interactions

Background:

  • Understanding cell adhesion dynamics is crucial for biological processes.
  • Quantifying molecular interactions at the pico-Newton scale presents significant technical challenges.

Purpose of the Study:

  • To develop and utilize novel vertical magnetic tweezers for precise force application.
  • To investigate the mechanical properties of vesicle-E-selectin bonds under controlled force.
  • To determine if vesicle shape analysis can quantify the number of specific bonds.

Main Methods:

  • Development of "vertical" magnetic tweezers for pico and sub-pico Newton force control.
  • Application of point-like forces to vesicles adhered via sialyl LewisX and E-selectin specific bonds.

Related Experiment Videos

  • Measurement of bound vesicle area and shape changes under varying forces.

    • Main Results:

    • Observed an exponential decrease in bound vesicle area, with a decay rate independent of applied force and system composition.
    • Measured an equilibrium state under force, characterized by increased binding at the center of the contact zone.
    • Demonstrated that vesicle shape determination can potentially quantify the number of specific bonds formed.

    Conclusions:

    • The developed magnetic tweezers enable precise force measurements in the pico-Newton range.
    • Vesicle-E-selectin bond strength and dynamics are force-insensitive within the tested range.
    • Vesicle shape analysis offers a promising method for determining the number of specific molecular bonds.