Related Experiment Video
Updated: Aug 11, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
Published on: November 16, 2019
Analysis of cellular structure by light scattering measurements in a new cytometer design based on a liquid-core
1Department of Electrical & Computer Engineering, University of Alberta, Edmonton, Canada.
Abstract:
The results of applying a novel microfluidic optical cytometer to generate and observe the light scattered from biological cells over a wide range of angles are presented. This cytometer incorporates a waveguide that increases the intensity of the scattered light to the extent that an inexpensive digital camera can be used to detect the light over a large solid angle. This device was applied to yeast cells and latex beads and experimental data were compared with the results of a finite difference time-domain (FDTD) method of simulation. The simulated scattering patterns were calculated from reported values of optical parameters and are in good qualitative agreement with experiment. It is demonstrated that this system could be used to acquire information on the microstructure and potentially the nanostructure of cells.
More Related Videos
08:32Design and Building of a Customizable, Single-Objective, Light-Sheet Fluorescence Microscope for the Visualization of Cytoskeleton Networks
Published on: January 26, 2024
11:34Controlled Synthesis and Fluorescence Tracking of Highly Uniform Poly(N-isopropylacrylamide) Microgels
Published on: September 8, 2016
Related Concept Videos
Flow Cytometry
In...
Confocal Fluorescence Microscopy
Three-Dimensional Microscopy in Microbiology