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Related Experiment Videos

Nanoscale surface engineered living cells with extended substrate spectrum.

W C Mak1, K W Sum, D Trau

  • 1Department of Chemistry and Sino-German Nano-Analytical Lab (SiGNAL), The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR.

IEE Proceedings. Nanobiotechnology
|February 16, 2006
PubMed
Summary

Scientists engineered yeast cells using Layer-by-Layer (LbL) technology to expand their capabilities. This surface engineering process enhanced the yeast

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Area of Science:

  • Biotechnology
  • Synthetic Biology
  • Microbial Engineering

Background:

  • Living microorganisms have limited substrate utilization capabilities.
  • Surface engineering offers a route to enhance microbial functions.
  • Layer-by-Layer (LbL) technology provides a versatile platform for nanoscale assembly on cell surfaces.

Purpose of the Study:

  • To engineer the surface of the yeast Arxula adeninivorans LS3 (Arxula) using LbL technology.
  • To extend the substrate spectrum of Arxula by introducing new enzymatic functions.
  • To evaluate the stability of the engineered cell surface and enzyme conjugates.

Main Methods:

  • Utilized Layer-by-Layer (LbL) assembly to encapsulate Arxula cells with polyelectrolyte and enzyme layers.
  • Incorporated lactate oxidase enzyme into the outer layer of the capsule.

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  • Assessed the retention of biological activity post-encapsulation.
  • Investigated the stability of the polymeric capsule and enzyme conjugates.

    • Main Results:

    • Successful encapsulation of Arxula cells using LbL technology while preserving biological activity.
    • Engineered Arxula demonstrated the ability to convert lactate to pyruvate via immobilized lactate oxidase.
    • Extended the substrate utilization range of Arxula.
    • Demonstrated stability of the engineered cell surface and enzyme linkages.

    Conclusions:

    • LbL technology is effective for surface engineering of living yeast cells.
    • Surface-functionalized Arxula exhibits enhanced metabolic capabilities by extending its substrate spectrum.
    • The engineered system offers a stable platform for developing novel biocatalytic microorganisms.