Variation in thickness of the large cryosections cut for whole-body autoradiography

A method to assess variation in thickness of the large cryosections for whole-body autoradiography (WBARG) was described, and the degree of intraslice and interslice variations were determined for our cryomicrotome system (LKB PMV-2250). Intraslice variation in thickness of the 180 x 80 mm cryosection was 0.72-0.92 micron within the range of section thickness for WBARG (15-50 microns), and interslice variation was 0.89-1.21 microns. These potential variations in section thickness should be kept in mind whenever working with quantitative WBARG.